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I ejakul\u00e1t, kter\u00fd je dle norem Sv\u011btov\u00e9 zdravotnick\u00e9 organizace (WHO) naprosto v\u00a0po\u0159\u00e1dku, obsahuje a\u017e 96 % spermi\u00ed, kter\u00e9 jsou abnorm\u00e1ln\u00ed a nevhodn\u00e9 pro oplozen\u00ed vaj\u00ed\u010dka. Na\u0161\u00edm \u00fakolem je odd\u011blit kvalitn\u00ed spermie, a z\u00a0nich vybrat ty nejlep\u0161\u00ed.<\/p>\n
Pou\u017e\u00edv\u00e1me metody, kter\u00e9 jsou \u0161etrn\u00e9 a z\u00e1rove\u0148 umo\u017e\u0148uj\u00ed zisk spermi\u00ed, kter\u00e9 jsou vzhledov\u011b v\u00a0po\u0159\u00e1dku, jsou vysoce pohybliv\u00e9, a nav\u00edc je u nich i v\u00fdrazn\u011b ni\u017e\u0161\u00ed riziko po\u0161kozen\u00ed jejich DNA. Pr\u00e1v\u011b pou\u017eit\u00ed selek\u010dn\u00ed metody pro v\u00fdb\u011br spermi\u00ed s\u00a0nepo\u0161kozenou DNA je velmi d\u016fle\u017eit\u00e9, nebo\u0165 DNA a jej\u00ed p\u0159\u00edpadn\u00e9 po\u0161kozen\u00ed nelze pozorovat pod sv\u011bteln\u00fdm mikroskopem, a to ani s\u00a0nejlep\u0161\u00edm vybaven\u00ed a p\u0159i nejv\u011bt\u0161\u00edm mo\u017en\u00e9m zv\u011bt\u0161en\u00ed. Z\u00e1rove\u0148 spermie s po\u0161kozenou DNA m\u016f\u017ee m\u00edt schopnost oplodnit vaj\u00ed\u010dko, nicm\u00e9n\u011b tato skute\u010dnost m\u016f\u017ee m\u00edt negativn\u00ed dopad na n\u00e1sledn\u00fd v\u00fdvoj embrya.<\/p>\n
Selekce spermi\u00ed je tedy d\u016fle\u017eit\u00e1 pro dosa\u017een\u00ed \u00fasp\u011b\u0161n\u00e9ho oplodn\u011bn\u00ed vaj\u00ed\u010dka, pro kvalitn\u00ed v\u00fdvoj embrya a t\u00edm vlastn\u011b pro cel\u00fd pr\u016fb\u011bh t\u011bhotenstv\u00ed.<\/p>\n
Na z\u00e1klad\u011b znalost\u00ed z\u00edskan\u00fdch ze sou\u010dasn\u00e9 odborn\u00e9 literatury i z\u00a0vlastn\u00edch zku\u0161enost\u00ed doporu\u010dujeme metodu selekce spermi\u00ed s\u00a0mikrofluidn\u00edmi \u010dipy jako optim\u00e1ln\u00ed metodu v\u00fdb\u011bru kvalitn\u00edch spermi\u00ed.<\/p>\n
Metoda je ke spermi\u00edm velice \u0161etrn\u00e1, nebo\u0165 nevy\u017eaduje \u017e\u00e1dnou p\u0159edchoz\u00ed \u00fapravu vzorku a sni\u017euje tak oxidativn\u00ed stres spermi\u00ed zp\u016fsoben\u00fd voln\u00fdmi radik\u00e1ly, ke kter\u00e9mu m\u016f\u017ee doch\u00e1zet p\u0159i standardn\u00ed p\u0159\u00edprav\u011b spermi\u00ed, kdy jsou spermie selektov\u00e1ny centrifugac\u00ed p\u016fsoben\u00edm odst\u0159ediv\u00e9 s\u00edly. Naopak je zde v\u00fdb\u011br umo\u017en\u011bn napodoben\u00edm p\u0159irozen\u00e9ho prost\u0159ed\u00ed ve vejcovodech. Spermie proch\u00e1zej\u00ed soustavou mikrofluidn\u00edch membr\u00e1n a do koncov\u00e9 \u010d\u00e1sti \u010dipu, odkud vyt\u0159\u00edd\u011bn\u00e9 spermie odeb\u00edr\u00e1me, se dost\u00e1vaj\u00ed jen ty rychle pohybliv\u00e9 a bez v\u00fdrazn\u00fdch tvarov\u00fdch odchylek. Takto vyt\u0159\u00edd\u011bn\u00e9 spermie tak vykazuj\u00ed men\u0161\u00ed mno\u017estv\u00ed vzhledov\u00fdch abnormalit, maj\u00ed v\u00fdrazn\u011b lep\u0161\u00ed pohyblivost, \u017eivotaschopnost a v\u00fdrazn\u011b ni\u017e\u0161\u00ed po\u0161kozen\u00ed DNA.<\/p>\n
Metoda MACS je zalo\u017eena na v\u00fdb\u011bru kvalitn\u00edch spermi\u00ed pomoc\u00ed magnetick\u00fdch nano\u010d\u00e1stic. Metoda, podobn\u011b jako pou\u017eit\u00ed mikrofluidn\u00edch \u010dip\u016f, zaji\u0161\u0165uje v\u00fdb\u011br spermi\u00ed, kter\u00e9 vykazuj\u00ed men\u0161\u00ed mno\u017estv\u00ed vzhledov\u00fdch abnormalit, maj\u00ed lep\u0161\u00ed pohyblivost, \u017eivotaschopnost a ni\u017e\u0161\u00ed po\u0161kozen\u00ed DNA.<\/p>\n
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Metody v\u00fdb\u011bru spermi\u00edI ejakul\u00e1t, kter\u00fd je dle norem Sv\u011btov\u00e9 zdravotnick\u00e9 organizace (WHO) naprosto v\u00a0po\u0159\u00e1dku, obsahuje a\u017e 96 % spermi\u00ed, kter\u00e9 jsou abnorm\u00e1ln\u00ed a nevhodn\u00e9 pro oplozen\u00ed vaj\u00ed\u010dka. Na\u0161\u00edm \u00fakolem je odd\u011blit kvalitn\u00ed spermie, a z\u00a0nich vybrat ty nejlep\u0161\u00ed. Pou\u017e\u00edv\u00e1me metody, kter\u00e9 jsou \u0161etrn\u00e9 a z\u00e1rove\u0148 umo\u017e\u0148uj\u00ed zisk spermi\u00ed, kter\u00e9 jsou vzhledov\u011b v\u00a0po\u0159\u00e1dku, jsou vysoce pohybliv\u00e9, […]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"I ejakul\u00e1t, kter\u00fd je dle norem Sv\u011btov\u00e9 zdravotnick\u00e9 organizace (WHO) naprosto v\u00a0po\u0159\u00e1dku, obsahuje a\u017e 96 % spermi\u00ed, kter\u00e9 jsou abnorm\u00e1ln\u00ed a nevhodn\u00e9 pro oplozen\u00ed vaj\u00ed\u010dka. Na\u0161\u00edm \u00fakolem je odd\u011blit kvalitn\u00ed spermie, a z\u00a0nich vybrat ty nejlep\u0161\u00ed.\r\n\r\nPou\u017e\u00edv\u00e1me metody, kter\u00e9 jsou \u0161etrn\u00e9 a z\u00e1rove\u0148 umo\u017e\u0148uj\u00ed zisk spermi\u00ed, kter\u00e9 jsou vzhledov\u011b v\u00a0po\u0159\u00e1dku, jsou vysoce pohybliv\u00e9, a nav\u00edc je u nich i v\u00fdrazn\u011b ni\u017e\u0161\u00ed riziko po\u0161kozen\u00ed jejich DNA. Pr\u00e1v\u011b pou\u017eit\u00ed selek\u010dn\u00ed metody pro v\u00fdb\u011br spermi\u00ed s\u00a0nepo\u0161kozenou DNA je velmi d\u016fle\u017eit\u00e9, nebo\u0165 DNA a jej\u00ed p\u0159\u00edpadn\u00e9 po\u0161kozen\u00ed nelze pozorovat pod sv\u011bteln\u00fdm mikroskopem, a to ani s\u00a0nejlep\u0161\u00edm vybaven\u00ed a p\u0159i nejv\u011bt\u0161\u00edm mo\u017en\u00e9m zv\u011bt\u0161en\u00ed. Z\u00e1rove\u0148 spermie s po\u0161kozenou DNA m\u016f\u017ee m\u00edt schopnost oplodnit vaj\u00ed\u010dko, nicm\u00e9n\u011b tato skute\u010dnost m\u016f\u017ee m\u00edt negativn\u00ed dopad na n\u00e1sledn\u00fd v\u00fdvoj embrya.\r\n\r\nSelekce spermi\u00ed je tedy d\u016fle\u017eit\u00e1 pro dosa\u017een\u00ed \u00fasp\u011b\u0161n\u00e9ho oplodn\u011bn\u00ed vaj\u00ed\u010dka, pro kvalitn\u00ed v\u00fdvoj embrya a t\u00edm vlastn\u011b pro cel\u00fd pr\u016fb\u011bh t\u011bhotenstv\u00ed.\r\n