Methods and techniques
We accept your wishes, approach the treatment with care and always want to choose only methods absolutely essential to the further outcome you seek.
If you want to have the best cultivation conditions for your embryos, choose the cultivation in the EmbryoScope +.
For the successful development of embryos in the laboratory, the stability of the conditions is of the most importance. With the development of new technologies, the old system of cultivation of embryos is replaced by cultivation in so-called time-lapse systems that closely monitor and record the development of embryos without the need to be removed from the ideal cultivation conditions of the incubator.
Our clinic is one of the first in the Czech Republic which have a new EmbryoScope + incubator, which has up to twice its capacity if compared to other time-lapse systems. So we are able to provide the best cultivation conditions for the embryos of all our patients. In addition, EmbryoScope + is designed for separate cultivation – each single-bar coded culture dish contains embryos of one couple and is isolated from another embryos so that when manipulating with one dish, embryo cultivation of other patients in the system is not impaired. In addition, a detailed record of embryo development allows our embryologists enough time and information to select the embryo with the highest development potential.
EmbryoGlue has the basic composition of a rich blastocyst culture medium and contains a high concentration of hyaluronan and recombinant human albumin. It is uniquely developed to mimic the conditions in the female uterus in order to help embryos implant after transfer.
Simply put – EmbryoGlue helps to glue the fertilized egg to the uterine wall. EmbryoGlue demonstrably increases chances of getting pregnant.
Selection methods of the “best sperm”
a) PICSI (preselected intracytoplasmatic sperm injection)
The so-called pre-selection method involves putting the sperm on a surface similar to the surface of the egg. They are observed and those with the greatest “adherence to this surface” are selected for fertilisation. Their fertilisation potential is considered to be the best.
b) MACS test
Selection of live sperm with undamaged DNA using magnetic nanoparticles. This method ensures that the damaged sperm is not used for fertilising the egg/s.
Sperm with damaged DNA may be capable of fertilising the egg but the embryo development may not be quite optimal. The sperm DNA damage is not visible. It cannot be determined by the shape or properties of the sperm in the microscope even when high magnification is used.
However, the MACS method can discover sperm which contain such damaged DNA. What is more, it can select these sperm and separate them from the rest. We then use only the sperm with undamaged DNA for the fertilisation, or in other words sperm with a significantly higher chance of fertilising the egg, starting the embryotic development and the whole course of the pregnancy.
c) Fertile Microfluidic Sperm Sorting Chip
Chemical-free (does not require any previous processing of sperm sample or use of devices that increase oxidative stress in sperm) method of sorting sperm using a disposable chip.
The method is based on the principle of natural sperm selection in a passage through micro-barriers imitating natural environment of female reproductive system. Selected sperm cells have better morphology, genetic quality and more than double viability and motility than unsorted sperm.
The sperm sample is pipetted into the inlet where the present sperm must actively migrate in the outlet against the movement of the fluid as well as through the above-mentioned micro-barriers. Sperm cells are sorted by separating healthy motile sperm in the outlet from poor-quality sperm captured in the duct.